Wolfgang René Hess

Wolfgang René Hess (* 8. Februar 1961) ist ein deutscher Professor für experimentelle Bioinformatik an der Fakultät für Biologie der Albert-Ludwigs-Universität Freiburg.

Wolfgang R. Hess

Biographie

Wolfgang R. Hess studierte von 1982 bis 1987 Biologie and den Universitäten von Rostock und Berlin an welcher auch Promovierte. Wolfgang Hess habilitierte 1999 an der Humboldt-Universität zu Berlin mit der Arbeit Untersuchungen zur Expression Licht- und Gewebsspezifisch regulierter Gene in höheren Pflanzen. Nach vier Jahren als wissenschaftlicher Oberassistent an der Humboldt-Universität Berlin ging er 2000 an das Massachusetts Institute of Technology und wurde 2003 Gründungsdirektor der Ocean Genome Legacy Foundation, einer privat finanzierte Stiftung zur Genomforschung der Biotechnologiefirma New England Biolabs. 2004 erhielt er den Ruf als Professor für Experimentelle Bioinformatik an der Universität Freiburg, wo er noch heute forscht und lehrt. Wolfgang R. Hess ist der Sprecher des Freiburger FORSYS (Forschungseinheiten der Systembiologie) Forschungszentrums FRISYS [1].

Forschung

Die Arbeitsgruppe von Professor Hess befasst sich mit Fragen der funktionellen Genomanalyse auf den Gebieten der Photosynthese und der Steuerung der Transkription und RNA-Prozessierung in prokaryotischen Organismen (Bakterien). Ein Schwerpunkt liegt dabei auf der Gruppe der Cyanobakterien. Diese Untersuchungen werden ergänzt durch Untersuchungen an den genetischen Systemen der Chloroplasten und Mitochondrien, insbesondere der Gerste (Hordeum vulgare L.).
Die Resultate dieser Untersuchungen haben Eingang in 60 Publikationen gefunden.
Als Gündungsdirektor der Ocean Genome Legacy Foundation in Boston von 2003-2004 hat Professor Wolfgang Hess wesentlich zur Strategie dieser neuen Forschungseinrichtung auf dem Gebiet der marinen Mikrobiologie und Genomforschung mariner Organismen insgesamt beigetragen.
Als Professor für Experimentelle Bioinformatik befassen sich er und seine Arbeitsgruppe seit 2004 verstärkt mit der post-genomischen Analyse prokaryotischer Organismen. Es besteht ein starkes Interesse daran, die an Modellorganismen entwickelten Methoden in großem Umfang auf Prokaryoten mit Relevanz für Medizin, Biotechnologie und Umwelt anzuwenden. Ein zunehmend wichtiger Aspekt ist in diesem Kontext der Einsatz systembiologischer Ansätze und Methoden.

Veröffentlichungen

Forschungsartikel

• 1991: Hess W.R., Schendel R., Börner T., Rüdiger W., (1991): Reduction of mRNA level for two nuclear encoded light regulated genes in the barley mutant albostrians is not correlated with phytochrome content and activity. J. Plant Physiology 138, 292-298.
• 1992: Hess W.R., Blank-Huber M., Fieder B., Börner T., Rüdiger W., (1992): Chlorophyll synthetase and chloroplast tRNAglu are present in heat-bleached, ribosome-deficient plastids. J. Plant Physiology 139, 427-430.

• Hess W.R., Schendel R., Rüdiger W., Fieder B., Börner T., (1992): Components of chlorophyll biosynthesis in a barley albina mutant unable to synthesize *-aminolevulinic acid by utilizing the transfer RNA for glutamic acid. Planta 188, 19-27.

• 1993: Hess W.R., Prombona A., Fieder B., Subramanian A.R., Börner T., (1993): Chloroplast rps15 and the rpoB/C1/C2 gene cluster are strongly transcribed in ribosome-deficient plastids: evidence for a functioning non-chloroplast-encoded RNA polymerase. EMBO J. 12, 563-571.

• Zeltz P., Hess W.R., Neckermann K., Börner T., Kössel H. (1993): Editing of the rpoB transcript is independent of chloroplast translation and shows different patterns in barley and maize. EMBO J. 12, 4291-4296.

• 1994: Hess W.R., Müller A., Nagy F., Börner T. (1994): Ribosome-deficient plastids affect transcription of light-induced nuclear genes: genetic evidence for a plastid-derived signal. Mol. Gen. Genet. 242, 305-312.

• Hess W.R., Hoch B., Zeltz P., Hübschmann T., Kössel H., Börner T. (1994): Inefficient rpl2 splicing in barley mutants with ribosome-deficient plastids. Plant Cell 6, 1455-1465.
• Zubko M.K., Zubko E.I., Fisahn J., Hess W.R., Börner T., Schieder O., Gleba Y.Y. (1994): Development-associated nucleo-cytoplasmic incompatibility in alloplasmic cybrids of Nicotiana tabacum (+Hyoscyamus niger). Proc. Russ. Acad. Sci. 337, 693-696.

• 1995: Lorenz M., Partensky F., Börner T., Hess W.R. (1995): Sequencing of RAPD fragments amplified from the genome of the prokaryote Prochlorococcus marinus. Biochem. Mol. Biol. Internat. 36, 705-713.

• Hess W.R., Weihe A., Loiseaux-De Goër S., Partensky F., Vaulot D. (1995): Characterization of the single psbA gene of Prochlorococcus marinus CCMP 1375 (Prochlorophyta). Plant Mol. Biol. 27, 1189-1196.

• 1996: Scanlan D.J., Hess W.R., Partensky F., Scanlan J., Vaulot D. (1996): High degree of genetic variation in Prochlorococcus (Prochlorophyta) revealed by RFLP analysis. Eur. J. Phycol. 31, 1-9.

• Hübschmann T., Hess W.R., Börner T. (1996): Impaired splicing of the rps12 transcripts in ribosome-deficient plastids. Plant Mol. Biol. 30, 109-123.
• Hess W.R., Partensky F., van der Staay G.W.M., Garcia-Fernandez J.M., Börner T., Vaulot D. (1996): Coexistence of phycoerythrin and a chlorophyll a/b antenna in a marine prokaryote. Proc. Natl. Acad. Sci. USA 93, 11126-11130.

• 1997: Vogel J., Hess W.R., Börner T. (1997): Precise branch point mapping and quantification of splicing intermediates. Nucleic Acids Res. 25, 2030-2031.

• Vogel J., Hübschmann T., Börner T., Hess W.R. (1997): Intron-internal RNA editing and splicing of trnK-matK precursor transcripts in barley plastids: support for MatK as an essential splice factor. J. Mol. Biol. 270, 179-187.
• Hess W.R., Börner T. (1997): Molecular Cloning of a DNA-binding protein from barley related to maize MNB1b and HMGa. Endocytobios. Cell Res., 12, 95-98.

• 1998: Richter S., Hess W.R., Krause M., Messer W. (1998): Unique organization of the dnaA region from Prochlorococcus marinus CCMP1375, a marine cyanobacterium. Mol. Gen. Genet. 257, 534-541.

• Hess W.R., Golz R., Börner, T. (1998): Analysis of randomly selected cDNAs reveals the expression of stress- and defence-related genes in the barley mutant albostrians. Plant Science 133, 191-201.
• Hess W.R., Fingerhut C., Schön A. (1998): RNase P RNA from Prochlorococcus marinus: Contribution of substrate domains to recognition by a cyanobacterial ribozyme. FEBS Lett. 431, 138-142.
• García-Fernández J.M., Hess W.R., Houmard J., Partensky F. (1998): Expression of the psbA gene in the marine oxychlorobacteria Prochlorococcus spp. Arch. Biophys. Biochem. 359, 17-23.

• 1999: Lokstein H., Steglich C., Hess W.R. (1999): Light-harvesting function of phycoerythrin in Prochlorococcus marinus. Bioch. Biophys. Acta 1410, 97-98.

• Hess W.R., Steglich C., Lichtlé C., Partensky F. (1999): Phycoerythrins of the oxyphotobacterium Prochlorococcus marinus are associated to the thylakoid membranes and are encoded by a single large gene cluster. Plant Mol. Biol. 40, 507-521.
• Vogel J., Börner T., Hess W.R. (1999): Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants. Nucleic Acids Res. 27, 3866-3874.
• Churin Y., Hess W.R., Börner T. (1999): A family of RNA-binding proteins from barley (Hordeum vulgare L.). Curr. Genet. 36, 173-181.
• Hedtke B., Wagner I., Börner T., Hess W.R. (1999): Interorganellar crosstalk in higher plants: impaired chloroplast development affects mitochondrial gene and transcript levels. Plant J. 19, 635-644.
• Strehl B., Holtzendorff J., Partensky F., Hess W.R. (1999): A small and compact genome in the marine cyanobacterium Prochlorococcus marinus CCMP 1375: lack of an intron in the gene for tRNA(Leu)UAA and a single copy of the rRNA operon. FEMS Microbiol. Lett. 181, 261-266.
• Hess W.R., Börner T. (1999): Organellar RNA polymerases of higher plants. Int. Rev. Cytol. 190, 1-59.
• Partensky, F. Hess W.R., Vaulot D. (1999): Prochlorococcus, an important marine photosynthetic prokaryote. Mol. Microbiol. Rev. 63, 106-127.

• 2000: Garczarek L., Hess W.R., Holtzendorff J., van der Staay G.W.M., Partensky F. (2000): Multiplication of antenna genes as a major adaptation to low light in a prokaryote. Proc. Natl. Acad. Sci. USA 97, 4098-4101.

• Rippka R., Coursin T., Hess W.R., Lichtlé C., Scanlan D.J., Palinska K., Iteman I., Partensky F., Vaulot D., Tandeau de Marsac N., Houmard J., Herdman M. (2000): Prochlorococcus marinus Chisholm et al. 1992, subsp. nov. pastoris, strain PCC 9511, the first axenic chlorophyll a2/b2-containing cyanobacterium (Oxyphotobacteria). Internat. J. Syst. Evol. Microbiol. 50, 1833-1847.
• Penno S., Campbell L., Hess W.R. (2000): Presence of phycoerythrin in two strains of Prochlorococcus isolated from the sub-tropical North Pacific Ocean. J. Phycol. 36, 723-729.

• 2001: Steglich C., Behrenfeld M., Koblizek M., Claustre H., Penno S., Prasil O., Partensky F., Hess W. R. (2001): Nitrogen deprivation strongly affects Photosystem II but not phycoerythrin level in the divinyl-chlorophyll b-possessing cyanobacterium Prochlorococcus marinus. Bioch. Biophys. Acta 1503, 341-349.

  Holtzendorff J., Partensky F., Jacquet S., Bruyant F., Marie D., Garczarek L., Mary I., Vaulot D., Hess W.R. (2001): Diel expression of cell cycle-related genes in synchronized cultures of Prochlorococcus PCC 9511. J. Bacteriol. 183, 915-922.

  Vogel J., Hess W.R. (2001): Complete 5’ and 3’ end maturation of group II intron containing tRNA precursors. RNA 7, 285-292.

  Garczarek L., Partensky F., Irlbacher H., Holtzendorff J., Babin M., Mary I., Thomas J.C., Hess W.R. (2001): Differential expression of antenna and core genes in Prochlorococcus PCC 9511 grown under a modulated light-dark cycle. Environ. Microbiol. 3, 168-175.

  Garczarek L., van der Staay G.W.M., W. R. Hess, Legall F. and Partensky F. (2001): Expression and phylogeny of the multiple antenna genes of the low-light adapted strain Prochlorococcus SS120 (Oxyphotobacteria). Plant Mol. Biol. 46, 683-693.

  Hess W.R., Rocap G., Ting C., Larimer F., Lamerdin J., Stilwagon S., Chisholm S.W. (2001): The photosynthetic apparatus of Prochlorococcus: Insights through comparative genomics. Photosynth. Res. 70, 53-72.

• 2002: Schön A., Fingerhut C., Hess W.R. (2002): Conserved and variable domains within divergent RNase P RNA gene sequences of Prochlorococcus spp. Internat. J. Syst. Evol. Microbiol. 52, 1383-1389.

• Holtzendorff J., Marie D., Post A.F., Partensky F., Rivlin A., Hess W.R. (2002): Synchronized expression of ftsZ in natural Prochlorococcus populations of the Red Sea. Environ. Microbiol. 4, 644-653.

• 2003: Steglich C., Post A.F., Hess W.R. (2003) Analysis of natural populations of Prochlorococcus spp. in the northern Red Sea using phycoerythrin gene sequences. Environ. Microbiol. 5, 681-690.

• Stickforth P., Steiger S., Hess W.R., Sandmann G. (2003) A novel type of lycopene  cyclase in the marine cyanobacterium Prochlorococcus marinus MED4. Arch. Microbiol. 179, 409-415.
• Vogel J., Axmann I.M., Herzel H., Hess W.R. (2003) Experimental and computational analysis of transcriptional start sites in the cyanobacterium Prochlorococcus MED4. Nucleic Acids Res. 31, 2890-2899.
• Steglich C., Mullineaux C.W., Teuchner K., Hess W.R., Lokstein H. (2003) Photophysical properties of Prochlorococcus marinus SS120 divinyl chlorophylls and phycoerythrin in in vitro and in vivo. FEBS Lett. 553, 79-84.
• Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I., Barbe V., Duprat S., Galperin M., Koonin, E.V., Le Gall F., Makarova K.S., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Vaulot D., Weissenbach J., Wincker P., Wolf Y.I. and Hess W.R. (2003) Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome. Proc. Natl. Acad. Sci. USA 100, 10020-10025.
• Rocap G., Larimer F.W., Lamerdin J., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M, Hauser L., Hess W.R., Johnson Z.I., Land M., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C., Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E., Chisholm S.W. (2003) Genome divergence in two Prochlorococcus ecotypes reflects Oceanic niche differentiation. Nature 424, 1042-1047.

• 2004 Jain K., Langen G., Hess W.R., Börner T., Hückelhoven R., Kogel K.H. (2004) The white barley mutant albostrians that is supersusceptible to the hemibiotroph Bipolaris sorokiniana shows enhanced resistance to the biotroph Blumeria graminis f. sp. hordei S. Mol. Plant Microbe Interact. 17, 374-382.

• Steglich C., Kehr J., Hess W.R. (2004) Proteome analysis of marine cyanobacteria based on total genome information. Marine Biotechnol. 6, 483-487.
• Emanuel C., Weihe A., Graner A., Hess W.R., Börner T. (2004) Chloroplast development affects expression of phage-type RNA polymerases in barley leaves. Plant Journal 38, 460-472.
• Hess W.R. (2004) Genome analysis of marine photosynthetic microbes and their global role. Curr. Opin. Biotechnol. 15, 191-198.

• 2005: Steglich C., Frankenberg-Dinkel N., Penno S., Hess W.R. (2005) A green light-absorbing phycoerythrin is present in the high-light-adapted marine cyanobacterium Prochlorococcus sp. MED4. Environ. Microbiol. 7, 1611–1618.

• Axmann I.M., Kensche P., Kohl S., Vogel J., Herzel H., Hess W.R. (2005) Identification of cyanobacterial non-coding RNAs by comparative genome analysis. Genome Biol. 6, R73: 1-16.

• 2006: Tolonen A.C., Hess W.R., Liszt G.B., Chisholm S.W. (2006) Genetic manipulation of Prochlorococcus MIT9313: GFP expression on an RSF1010 plasmid and Tn5 transposition. Appl. Environ. Microbiol. 72, 7607-7613.

• U., Axmann I.M., Hess W.R., Wilde A. (2006) An internal antisense RNA regulates expression of the photosynthesis gene isiA. Proc. Natl. Acad. Sci. USA, 103, 7054-7058

• 2007: Axmann I.M., Holtzendorff J., Voss B., Kensche P., Hess W.R. (2007) Two distinct types of 6S RNA in Prochlorococcus. Gene, in press.

• Fattash I., Voss B., Reski R., Hess W.R., Frank W. (2007) Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution. BMC Plant Biol. 7:13
• Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Schneider I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Süssmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R. (2007) Comparative analysis of the complete genome sequence of the plant growth promoting Bacillus amyloliquefaciens FZB42. Nature Biotechnol. 25, in press.
• Lindell D., Jaffe J.D., Coleman M.L., Futschik, M., Axmann I.M., Rector T., Kettler G., Sullivan M.B., Steen R., Hess W.R., Church G.M., Chisholm S.W. (2007) Genome-wide expression dynamics of a marine virus and host reveal features of co-evolution. Nature, in press.

Buchkapitel und Übersichtsartikel

• 1993: Börner T., Hess W.R., (1993): Altered nuclear, mitochondrial and plastid gene expression in white barley cells containing ribosome-deficient plastids. In: Plant Mitochondria, Kück U., Brennicke A., (eds.), Verlag Chemie, Weinheim, Germany, pp. 207-220.
• 1994: Hess W.R., Hübschmann T., Börner T. (1994): Ribosome-deficient plastids of albostrians barley: extreme representatives of non-photosynthetic plastids. Endocytobios. Cell Res. 10, 65-80.
• 1997: Hess W.R., Linke B., Börner T. (1997): Effects of plastid differentiation on nuclear gene transcription. In: Intertaxonic Combination and Symbiotic Adaptation, Schenk, H.E.A., Herrmann, R.G., Jeon, K.W., Müllert, N.E., Schwemmler, W. (eds.), Springer Verlag, Heidelberg, Germany, pp. 233-242.
• 1998: Vogel J., Börner T., Hess W.R. (1998): Barley plastid genes encoding trnI-GAU and trnA-UGC are disrupted by group II introns (Accession No. AF072710; PGR98-164). Plant Physiol. 118, 331.

• Hess W.R., Börner T. (1998): Cloning and characterization of expression of cytosolic ascorbate peroxidase cDNA from barley (Accession No. AJ006358; PGR98-135). Plant Physiol. 118, 329.
• Hess W.R., Steglich C. (1998): Prochlorococcus marinus und das Geheimnis des tiefen Chlorophyllmaximums. Bioforum 12, 773-776.

• 1999: Börner, T., Hedtke, B., Hess W.R., Hübschmann, T., Legen, J., Herrmann, R.G., Weihe, A. (1999): Phage-type RNA polymerases in higher plants. In: The Chloroplast: from Molecular Biology to Biotechnology, Argyroudi-Akoyunoglou, J.H., Senger, H. (eds.), Kluwer Acad. Publishers, pp. 73-78.

• Hess W.R., Partensky F. (1999): Analysis of a phycobiliprotein gene cluster in Prochlorococcus marinus CCMP 1375: Identification of a putative linker polypeptide and the phylogeny of Prochlorococcus phycoerythrins. In: The Photosynthetic Prokaryotes, Peschek, G.A., Löffelhardt, W., Schmetterer G. (eds.), Plenum Press New York, USA. pp. 751-761.
• Hess W.R., Schön A. (1999): Characterization of rnpB, the gene encoding the RNA component of RNase P of Prochlorococcus marinus. In: Marine Cyanobacteria, Charpy, L., Larkum, A.W.D. (eds.). Bulletin de l'Institut Océanographique, Monaco, 19, pp. 77-82.

• 2005: Rediger A., Axmann I.M, Hess W.R. (2005) Analyzing the Prochlorococcus light-harvesting system: regulatory sequence elements and novel antisera. In: Photosynthesis: Fundamental Aspects to Global Perspectives.

• Voss B., Hess W.R. (2005) Genetik in einer neuen Dimension: Regulatorische RNA. Bioforum 10, 48-50.

• 2007: Hess W.R. (2007) Comparative genomics of marine cyanobacteria and their phages. In: Genomics and Molecular Biology of Cyanobacteria. Herrero A. and Flores E., eds. Horizon Scientific Press (Norwich, UK), 89-116.

Weblinks

• Homepage AG Hess
• FRISYS
• Stark und erfolgreich durch komparativen Ansatz in der Systembiologie
• Ocean Genome Legacy Foundation