Slippery sequence
A slippery sequence is a small section of codon nucleotide sequences (usually UUUAAAC) that controls the rate of ribosomal frameshifting. A slippery sequence causes a faster ribosomal transfer which in turn can cause the reading ribosome to "slip." This allows a tRNA to shift by 1 base after it has paired with its anticodon, changing the reading frame.[1][2][3][4][5]
The slippery nature of these sequences, like stop codon read-throughs, is related to pseudoknots forming at or downstream of the slippery site. The Gibbs free energy of these knots give a hint at how often frameshift happens.[6] A list of slippery sequences found in animal viruses is available from Huang et al.[7]
See also
- Nucleic acid tertiary structure
- Open reading frame
- Ribosomal frameshifting
- Translational frameshift
- Transposable element
References
- ^ Green L, Kim CH, Bustamante C, Tinoco I Jr. "Characterization of the Mechanical Unfolding of RNA Pseudoknots." J Mol Biol. 26 May 2007
- ^ Chien-Hung Yu, Mathieu H. M. Noteborn and René C. L. Olsthoorn.Stimulation of ribosomal frameshifting by antisense LNA. Nucl.Acids Res (2010) 38 (22):8277–8238
- ^ "Archived copy". Archived from the original on 2013-10-02. Retrieved 2013-07-28.
{{cite web}}: CS1 maint: archived copy as title (link) - ^ "Molecular Biology: Frameshifting occurs at slippery sequences". Molecularstudy.blogspot.com. Retrieved 2013-07-28.
- ^ Farabaugh, P. J.; Björk, G. R. (15 March 1999). "How translational accuracy influences reading frame maintenance". EMBO J. 18 (6): 1427–1434. doi:10.1093/emboj/18.6.1427. PMC 1171232. PMID 10075915.
- ^ Cao, S; Chen, SJ (11 March 2008). "Predicting ribosomal frameshifting efficiency". Physical biology. 5 (1): 016002. doi:10.1088/1478-3975/5/1/016002. PMC 2442619. PMID 18367782.
{{cite journal}}: CS1 maint: article number as page number (link) - ^ Huang, Xiaolan; Cheng, Qiang; Du, Zhihua (2013). "A Genome-Wide Analysis of RNA Pseudoknots That Stimulate Efficient −1 Ribosomal Frameshifting or Readthrough in Animal Viruses". BioMed Research International. 2013: 1–15. doi:10.1155/2013/984028.
External links
- Pseudobase
- Recode
- Frameshifting,+Ribosomal at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
- Wise2 - aligns a protein against a DNA sequence allowing frameshifts and introns
- FastY - compare a DNA sequence to a protein sequence database, allowing gaps and frameshifts
- Path - tool that compares two frameshift proteins (back-translation principle)
- Recode2 - Database of recoded genes, including those that require programmed Translational frameshift.
- Page for Coronavirus frameshifting stimulation element at Rfam
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