Methylation specific oligonucleotide microarray
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Methylation specific oligonucleotide microarray was developed as a technique to map methylation changes in DNA in cancer. This technique was developed by Professor Tim Hui-Ming Huang and was published in journal Genome Research on 2002 (Gitan et al., 2002). The method utilizes bisulfite-modified DNA that is used as templates for PCR amplification, which is subsequently hybridized to oligonucleotide microarray.
Implications on Cancer Research
Cancer cells often develop atypical methylation patterns, at CpG sites in promoters of tumour suppressor genes. High levels of methylation at a promoter leads to downregulation of the corresponding genes and is characteristic of carcinogenesis. It is one of the most consistent changes observed in early stage tumour cells.[1] Methylation specific oligonucleotide microarray allows for the high resolution and high throughput detection of numerous methylation events on multiple gene promoters.
External links
- Resources, information and specific protocols for DNA Methylation Analysis
- Software for DNA Methylation Analysis
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- ^ Gitan, Raad S.; Shi, Huidong; Chen, Chuan-Mu; Yan, Pearlly S.; Huang, Tim Hui-Ming (2002). "Methylation-Specific Oligonucleotide Microarray: A New Potential for High-Throughput Methylation Analysis". Genome Research. 12 (1): 158–164. doi:10.1101/gr.202801. ISSN 1088-9051. PMID 11779841.