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DNA barcoding in diet assessment
DNA barcoding is broadly used to analyse the diet of both invertebrate and vertebrate organisms[1][2] and further detect and characterize their trophic interactions[3][4].
Mammal diet assessment
General intro sentence on use of DNA barcoding in mammals diet assessment
Methods
Describe methods and highlight main approaches depending on previous knowledge on diet: use of specific or generic markers.
Technical details: target regions for mammals (DNA barcodes, cytb); blocking primers of the host (Shehzad et al., 2012; Vestheim & Jarman, 2008; King et al., 2008; Shehzad et al., 2012).
Case studies
bear (De Barba et al., 2014)
leopard cat (Shehzad et al., 2012)
chamois (Rayé et al., 2011)
macaroni penguin (Deagle et al., 2007)
seal (Casper et al., 2007; Meheust et al., 2015)
red bat (Clare et al., 2009)
Comparison with traditional diet assessment approaches
Casper et al., 2007a-b; Mumma et al., 2015; Shores et al., 2015; Nielsen et al., 2018 review
Potentials and shortcomings
Bird diet assessment
Arthropod diet assessment
- ^ King, R. A.; Read, D. S.; Traugott, M.; Symondson, W. O. C. (2008-01-14). "INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA-based approaches: OPTIMIZING MOLECULAR ANALYSIS OF PREDATION". Molecular Ecology. 17 (4): 947–963. doi:10.1111/j.1365-294X.2007.03613.x.
- ^ Pompanon, Francois; Deagle, Bruce E.; Symondson, William O. C.; Brown, David S.; Jarman, Simon N.; Taberlet, Pierre (2012). "Who is eating what: diet assessment using next generation sequencing". Molecular Ecology. 21 (8): 1931–1950. doi:10.1111/j.1365-294X.2011.05403.x. ISSN 1365-294X.
- ^ Sheppard, S. K.; Harwood, J. D. (2005-10). "Advances in molecular ecology: tracking trophic links through predator-prey food-webs". Functional Ecology. 19 (5): 751–762. doi:10.1111/j.1365-2435.2005.01041.x. ISSN 0269-8463.
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(help) - ^ Erickson, David L.; Uriarte, Maria; García-Robledo, Carlos; Kress, W. John (2015-01-01). "DNA barcodes for ecology, evolution, and conservation". Trends in Ecology & Evolution. 30 (1): 25–35. doi:10.1016/j.tree.2014.10.008. ISSN 0169-5347. PMID 25468359.