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DNA barcoding in diet assessment

DNA barcoding is broadly used to analyse the diet of both invertebrate and vertebrate organisms (King et al., 2008; Pompanon et al., 2012) and further detect their trophic interactions (Sheppard & Harwood, 2005; Kress et al., 2015).

Mammal diet assessment

General intro sentence on use of DNA barcoding in mammals diet assessment

Methods

Describe methods and highlight main approaches depending on previous knowledge on diet: use of specific or generic markers.

Technical details: target regions for mammals (DNA barcodes, cytb); blocking primers of the host (Shehzad et al., 2012; Vestheim & Jarman, 2008; King et al., 2008; Shehzad et al., 2012).

Case studies

bear (De Barba et al., 2014)

leopard cat (Shehzad et al., 2012)

chamois (Rayé et al., 2011)

macaroni penguin (Deagle et al., 2007)

seal (Casper et al., 2007; Meheust et al., 2015)

red bat (Clare et al., 2009)

Comparison with traditional diet assessment approaches

Casper et al., 2007a-b; Mumma et al., 2015; Shores et al., 2015; Nielsen et al., 2018 review