DNA construct
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A DNA construct is an artificially constructed segment of nucleic acid that is going to be "transplanted" into a target tissue or cell. It often contains a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail.[1]
See also
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- ^ Liu, Chengyu (3 November 2013). "Strategies for Designing Transgenic DNA Constructs". Methods in Molecular Biology. 1027: 183–201. doi:10.1007/978-1-60327-369-5_8. Retrieved 7 September 2017.