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Random chimeragenesis on transient templates

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Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate(Coco et al. 2001).For example for increased rate and extent of biodesulfurization of diesel by modification of dibenzothiophene mono-oxygenase. DNA shuffling is a similar but less powerful method used in directed evolution experiments.

References

  • Coco, Wayne M.; Levinson, William E.; Crist, Michael J.; Hektor, Harm J.; Darzins, Aldis; Pienkos, Philip T.; Squires, Charles H.; Monticello, Daniel J. (31 March 2001). "DNA shuffling method for generating highly recombined genes and evolved enzymes". Nature Biotechnology. 19 (4): 354–359. doi:10.1038/86744. PMID 11283594.


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