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SSC buffer

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This is an old revision of this page, as edited by Ortho (talk | contribs) at 19:16, 24 May 2016 (Every person beginning to work with molecular biology will go to Wikipedia to look up SSC buffer. There has to be a page like this discussing it. SSC is not Citric acid by any stretch.). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

In biochemistry and molecular biology, the saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer.

A 20X stock solution consists of 3 M sodium chloride and 300 mM trisodium citrate (adjusted to pH 7.0 with HCl).

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