Multi-parametric surface plasmon resonance

Surface Plasmon Resonance (SPR) is an established method for biomolecular interaction analysis. It is a real-time label-free method. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is based on the same surface plasmon foundations, but it uses a different optical setup, a goniometric SPR setup. While MP-SPR provides same kinetic information as SPR (Equilibrium_constant, Dissociation_constant, Ligand_(biochemistry)#Receptor.2Fligand_binding_affinity), it provides also structural information (Refractive_index, layer thickness). The MP-SPR method is used to measure surface interactions and nanolayer properties.

History The goniometric SPR method was researched alongside focused beam SPR and Otto configurations at VTT Technical Research Centre of Finland since 1980s by Dr. Janusz Sadowski. The goniometric SPR optics was commercialized by Biofons Oy for use in point-of-care applications. Since 2006, it was developed by BioNavis Ltd and commercialized in 2008. In 2011, the MP-SPR was introduced when additional wavelengths were added and first thin film analysis were performed.

Principle

An incident beam of p-polarized light strikes an electrically conducting gold layer at the interface of a glass sensor with high RI (Refractive Index) and an external medium/sample with low RI. At a given angle, the excitation of surface plasmons takes place resulting in a reduced intensity of the reflected light, which can be seen as a dip in the SPR curve. In MP-SPR, the whole angular range is scanned automatically with multiple wavelengths providing information on molecular interaction kinetics and also on structure of the formed layer.

The MP-SPR optical setup measures at multiple wavelengths simultaneously (similarly to spectroscopic SPR), but instead of measuring at a fixed angle, it rather scans across a wide range of θ angles (for instance 40 degrees). This results in measurements of full SPR curves at multiple wavelenghts providing additional information about structure of the film.

Measured values The measured full SPR curves (x-axis: angle, y-axis: reflected light intensity) can be transcribed into sensograms (x-axis: time, y-axis: selected parameter such as peak minimum, light intensity, peak width). The sensograms can be fitted using binding models to obtain kinetic parameters including on- and off-rates and affinity. The full SPR curves are used to fit Fresnel equations to obtain thickness and refractive index of the layers.

Applications The method has been used in life sciences, material sciences and biosensor development. In life sciences, the main applications focus on pharmaceutical development including small molecule or antibody interactions with target with a biomembrane or with a living cell monolayer. As first in the world, MP-SPR is able to separate transcellular and paracellular drug uptake in real-time and label-free. In biosensor development, MP-SPR is used for assay development for point-of-care applications. Typical developed biosensors include electrochemical printed biosensors, ELISA and SERS. In material sciences, MP-SPR is used for optimization of thin solid films from Ångströms to 100 nanometers (graphene, metals, oxides), soft materials up to microns (nanocellulose, polyelectrolyte) including nanoparticles.