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Restriction enzyme mediated integration

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Restriction Enzyme Mediated Integration (abbreviated as REMI) is a technique for inserting DNA fragments (linear plasmid) into a genome site that has been cut using a certain restriction enzyme.

Mechanism

Applications

The REMI method has been used to generate genetically modified organisms.

Creating transgenic Xenopus

REMI has been used for large-scale transgenesis in Xenopus laevis embryos in order to identify in vivo requirements for FGF signaling during gastrulation[1].

Creating transgenic Mushrooms

References

  1. ^ Kroll, K. L.; Amaya, E. (1996-10-01). "Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation". Development (Cambridge, England). 122 (10): 3173–3183. ISSN 0950-1991. PMID 8898230.
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