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High-performance thin-layer chromatography

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Analysis of food-dyes: (A) photo of HPTLC plate (developed from both sides), (B) multi-wavelength scan of mix 1, (C) calibration function, (D) mass spectra of selected zones, (E) results[1]

High performance thin layer chromatography (HPTLC) is an enhanced form of thin layer chromatography (TLC). A number of enhancements can be made to the basic method of thin layer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements.

Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One recent approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample.[2]

The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography.[3] The procedure begins with development of sample loaded plate with first solvent. After removing it, the plate is rotated 90° and developed with a second solvent.

Instrumentation

Widely used instrument for HPTLC is from CAMAG,[4] Switzerland. It provides automated sample application (loading), plate development, detection and documentation.[5]

References

Notes
  1. ^ G. Morlock, C. Oellig (2009), CAMAG Bibliography Service 103, 5
  2. ^ Morlock, Gertrud E. (2010). "Miniaturized planar chromatography using office peripherals". Analytical Chemistry. 82 (7): 2940–2946. doi:10.1021/ac902945t. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  3. ^ Nurok, David (1989). "Strategies for optimizing the mobile phase in planar chromatography". Chemical Reviews. 89 (2): 363–375. doi:10.1021/cr00092a007.
  4. ^ http://www.camag.com/en/home.cfm
  5. ^ http://www.camag.com/en/tlc_hptlc/what_is_tlchptlc.cfm
Sources
  • Puri, A., Ahmad, A. and Panda, B. P. (2010), Development of an HPTLC-based diagnostic method for invasive aspergillosis. Biomed. Chromatogr., 24: 887–892. doi: 10.1002/bmc.1382 [1]
  • Ajaz Ahmad, M Mujeeb, Bibhu Prasad Panda (2010) An HPTLC Method for the Simultaneous Analysis of Compactin and Citrinin in Penicillium citrinum Fermentation Broth. Journal of Planar Chromatography-modern TLC 23 (4), 282–285 [2]

Bibliography

  • F. Geiss (1987): Fundamentals of thin layer chromatography planar chromatography, Heidelberg, Hüthig, ISBN 3-7785-0854-7
  • Jork, H., Funk, W., Fischer, W., Wimmer, H. (1990): Thin-Layer Chromatography: Reagents and Detection Methods, Volume 1a, VCH, Weinheim, ISBN 3-527-278834-6
  • Jork, H., Funk, W., Fischer, W., Wimmer, H. (1994): Thin-Layer Chromatography: Reagents and Detection Methods, Volume 1b, VCH, Weinheim
  • Hahn-Deinstorp, E. (2000): Applied Thin-Layer Chromatography. Best Practice and Avoidance of Mistakes, Wiley-VCH, Weinheim, ISBN 3-527-29839-8
  • Spangenberg, B., Poole, C. F., Weins, C. (2011): Quantitative Thin-Layer Chromatography : A Practical Survey, Springer, Berlin, Heidelberg
  • Zlatkis, A., Kaiser, RE. (editors), (1977): HPTLC : high performance thin-layer chromatography, Amsterdam, Elsevier


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