COMPLEAT (Bioinformatics tool)
Protein Complex Enrichment Analysis Tool (COMPLEAT) is an online tool to analyze high-throughput datasets (or small-scale datasets) using protein complex enrichment analysis. The tool uses a protein complex resource as the back end annotation data instead of conventional Gene Ontology- or pathway-based annotations. The tool incorporates several useful features in order to provide a comprehensive data-mining environment, including network-based visualization and interactive querying options.
COMPLEAT is useful to analyze RNAi screens, Proteomic datasets, Gene expression data and any other high-throughput datasets where protein complex information is relevant.
COMPLEAT was successfully applied to identify
1. Dynamic protein complexes regulated by insulin and epidermal growth factors signaling, including a role of Brahma complex in the cellular response to insulin[2].
2. Evolutionarily conserved molecular complexes that regulate nucleolar size when the complex constituents were targeted by RNA interference [3].
References
- ^ . Science Signaling http://stke.sciencemag.org/cgi/content/abstract/sigtrans;6/264/rs5.
{{cite web}}: Missing or empty|title=(help) - ^ Vinayagam, A (2013 Feb 26). "Protein complex-based analysis framework for high-throughput data sets". Science signaling. 6 (264): rs5. PMID 23443684.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help) - ^ Neumüller, R.A. (2013 Aug 20). "Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses". Science signaling. 6 (289): ra70.
{{cite journal}}: Check date values in:|date=(help); Unknown parameter|coauthors=ignored (|author=suggested) (help)