lin-4 microRNA precursor
| lin-4 microRNA precursor | |
|---|---|
 Predicted secondary structure and sequence conservation of lin-4 | |
| Identifiers | |
| Symbol | lin-4 |
| Rfam | RF00052 |
| miRBase | MI0000002 |
| miRBase family | MIPF0000303 |
| Other data | |
| RNA type | Gene; miRNA |
| Domain(s) | Eukaryota |
| GO | GO:0035195 GO:0035068 |
| SO | SO:0001244 |
| PDB structures | PDBe |
lin-4 is a microRNA (miRNA) that was identified from a study of developmental timing in the nematode Caenorhabditis elegans.[1][2] It was the first to be discovered of the miRNAs, a class of non-coding RNAs involved in gene regulation.[3] lin-4 is transcribed from autonomous miRNA promoters and is developmentally regulated, with lin-4 miRNA accumulation occurring at the L2 stage of post-embryonic development. Additional to this is the up-regulation of endogenous lin-4 primary transcripts upon appearance of the lin-4 mature form. [4] lin-4 is found on chromosome II in C. elegans and is complementary to sequences in the 3' untranslated region (UTR) of lin-14 mRNA. lin-4 represses accumulation of the LIN-14 protein.[5] lin-4 also targets lin-28 and reduces protein expression.[6] miRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. The extents of the hairpin precursors are not generally known and are estimated based on hairpin prediction. The products are thought to have regulatory roles through complete or partial complementarity to mRNA.
References
- ^ Lee RC, Feinbaum RL, Ambros V (1993). "The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14". Cell. 75 (5): 843–854. doi:10.1016/0092-8674(93)90529-Y. PMID 8252621.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Rougvie, AE (2001). "Control of developmental timing in animals". Nat Rev Genet. 2 (9): 690–701. doi:10.1038/35088566. PMID 11533718.
- ^ Ambros, V (2001). "microRNAs: tiny regulators with great potential". Cell. 107 (7): 823–826. doi:10.1016/S0092-8674(01)00616-X. PMID 11779458.
- ^ Balasubramanian C, Ojha RP, Maiti S, Desideri A (2010). "Sampling the structure of the noncanonical lin-4:lin-14 microRNA:mRNA complex by molecular dynamics simulations". J Phys Chem B. 114 (49): 16443–9. doi:10.1021/jp104193r. PMID 21090710.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Olsen PH, Ambros V (1999). "The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation". Dev. Biol. 216 (2): 671–80. doi:10.1006/dbio.1999.9523. PMID 10642801.
- ^ Bagga S, Bracht J, Hunter S; et al. (2005). "Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation". Cell. 122 (4): 553–63. doi:10.1016/j.cell.2005.07.031. PMID 16122423.
{{cite journal}}: Explicit use of et al. in:|author=(help)CS1 maint: multiple names: authors list (link)
External links
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