SSC buffer
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In biochemistry and molecular biology, the saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of [gels] during a vacuum transfer.
A 20X stock solution consists of 3 M sodium chloride and 300 mM trisodium citrate (adjusted to pH 7.0 with HCl).
See also
References
- Material safety data sheet for 20x SSC buffer, Mediatech, Inc.
- Molecular Cytogenetics and biology Buffers, University of Leicester
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