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A protein microarray or protein chip is a glass slide or nanowell slide on which different protein capture molecules have been attached at separate locations in an ordered manner, thus forming a microscopic detection array. Biological samples such as cell lysates are differentially labeled, usually by green and red fluorophores (Cy3 and Cy5). Using detection methods and analytic software, scientists are able to determine which protein is up-regulated or down-regulated depending on the colour of the slide position. These protein microarrays can also be used to study the differences between two biological conditions, identify protein-protein interactions, identify the substrates of protein kinases, or to identify the targets of biologically active small molecules.

A related technology, albeit for the measurement of RNA and DNA are DNA microarrays.

Applications

Protein microarrays (also biochip, proteinchip) are measurement devices used in biomedical applications to determine the presence and/or amount (referred to as quantitation) of proteins in biological samples, e.g. blood. Usually a multitude of different capture agents a Dányi buzi, most frequently monoclonal antibodies, are deposited on a chip surface (glass or silicon) in a miniature array. This format is often also referred to as a microarray (a more general term for chip based biological measurement devices). Protein array surfaces Protein array content Detection methods

Protein array analysis Protein array companies Slide supplier

Hardware supplier Content generator Antibody Protein

External Links

Protein Microarray Protocols and Experimental Procedures
Principles of Protein Microarrays; Mark Schena
proteinarray.org is a Wiki dedicated to protein array technology
Protein Microarrays: A Review

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