Pulse-chase analysis
In biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a radioactive compound (pulse) and then to the same compound in nonradioactive form (chase).
Uses
This method is useful for determining the activity of certain cells over a prolonged period of time. The method has been used to study protein kinase C, ubiquitin, and many other proteins.
References
- Pulse Chase Analysis of Protein Kinase C
- Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail
- Figures of Pulse-Chase Analysis
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