Electropherogram
An electropherogram, or electrophoregram, can also be referred to as an EPG or e-gram. It is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of forensic biology, molecular biology and biochemistry.[1] The method utilizes data points that correspond with a specific time and fluorescence intensity at various wavelengths of light to represent a DNA profile.[2]
In the field of genetics, an electropherogram is a plot of DNA fragment sizes, typically used for genotyping such as DNA sequencing.[3] The data is plotted with time on the x-axis and fluorescence intensity on the y-axis. Such plots are often achieved using an instrument such as an automated DNA sequencer paired with capillary electrophoresis (CE). Such electropherograms may be used to determine DNA sequence genotypes, or genotypes that are based on the length of specific DNA fragments or number of short tandem repeats (STR) at a specific locus by comparing the sample to internal size standards and allelic ladder data using the same size standard[4]. These genotypes can be used for:
See also
References
- ^ Karabiber, F (2013). "A peak alignment algorithm with novel improvements in application to electropherogram analysis". Journal of Bioinformatics and Computational Biology. 11 (5): 1350011. doi:10.1142/S021972001350011X. PMC 4529286. PMID 24131055.
- ^ Butler, J. M. (2015). Advanced topics in forensic Dna typing: Interpretation. Elsevier.
- ^ Schwartz, H. and Guttman, A., 1995. Separation of DNA by capillary electrophoresis. Beckman.
- ^ Butler, J. M. (2015). Advanced topics in forensic Dna typing: Interpretation. Elsevier.
External links
- PHPH — web-based tool for electropherogram quality analysis
- Systematic differences in electropherogram peak heights reported by different versions of the GeneScan Software
- DYS464 Electropherogram Interpretation Discrepancy with images
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