Electropherogram
An electropherogram, or electrophoregram, can also be referred to as an EPG or e-gram. It is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of forensic biology, molecular biology and biochemistry.[1] The method utilizes data points that correspond with a specific time and fluorescence intensity at various wavelengths of light to represent a DNA profile.[2]
In the field of genetics, an electropherogram is a plot of DNA fragment sizes, typically used for genotyping such as DNA sequencing.[3] The data is plotted with time on the x-axis and fluorescence intensity on the y-axis. Such plots are often achieved using an instrument such as an automated DNA sequencer paired with capillary electrophoresis (CE).[4] Such electropherograms may be used to determine DNA sequence genotypes, or genotypes that are based on the length of specific DNA fragments or number of short tandem repeats (STR) at a specific locus by comparing the sample to internal size standards and allelic ladder data using the same size standard[5]. These genotypes can be used for:
See also
References
- ^ Karabiber, F (2013). "A peak alignment algorithm with novel improvements in application to electropherogram analysis". Journal of Bioinformatics and Computational Biology. 11 (5): 1350011. doi:10.1142/S021972001350011X. PMC 4529286. PMID 24131055.
- ^ Butler, J. M. (2015). Advanced topics in forensic Dna typing: Interpretation. Elsevier.
- ^ Schwartz, H. and Guttman, A., 1995. Separation of DNA by capillary electrophoresis. Beckman.
- ^ Butler, J. M. (2015). Advanced topics in forensic Dna typing: Interpretation. Elsevier.
- ^ Butler, J. M. (2015). Advanced topics in forensic Dna typing: Interpretation. Elsevier.
External links
- PHPH — web-based tool for electropherogram quality analysis
- Systematic differences in electropherogram peak heights reported by different versions of the GeneScan Software
- DYS464 Electropherogram Interpretation Discrepancy with images
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