Restriction fragment mass polymorphism
Restriction Fragment Mass Polymorphism (RFMP) is a technology which digests DNA into oligonucleotide fragments, and detects variation of DNA sequences by molecular weight of the fragments. RFMP is a proprietary technology of GeneMatrix and can be utilized for genotyping viruses and microorganisms, and for human genome research.
Overview
Restriction fragment mass polymorphism (RFMP) is an application of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF), used for identifying individual nucleotides from a DNA fragment, most commonly used in labeling single nucleotide polymorphisms (SNP). RFMP was developed as a successor to the similar restriction fragment length polymorphism (RFLP) with the intent to allow for more SNPs. Rather than read out lengths of fragments as RFLP does, the individual nucleotides are read out using MALDI-TOF, which gives specific clarity over same-length site cutting[1].
References
- Kim, Y (2005). "Population Genotyping of Hepatitis C Virus by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Short DNA Fragments", Clinical Chemistry, 51: 1123-1131
External links
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- ^ Hong, Sun Pyo; Ji, Seung Il; Rhee, Hwanseok; Shin, Soo Kyeong; Hwang, Sun Young; Lee, Seung Hwan; Lee, Soong Deok; Oh, Heung-Bum; Yoo, Wangdon; Kim, Soo-Ok (2008-06-09). "A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry". BMC genomics. 9: 276. doi:10.1186/1471-2164-9-276. ISSN 1471-2164. PMC 2442615. PMID 18538037.
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