Plaque reduction neutralization test
The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus.Cite error: A <ref> tag is missing the closing </ref> (see the help page).
The concentration of serum to reduce the number of plaques by 50% compared to the serum free virus gives the measure of how much antibody is present or how effective it is. This measurement is denoted as the PRNT50 value.
Currently it is considered to be the "gold standard" for detecting and measuring antibodies that can neutralise the viruses that cause many diseases.[1][2] It has a higher sensitivity than other tests like hemagglutination and many commercial Enzyme immunoassay without compromising specificity. Moreover, it is more specific than other serological methods for the diagnosis of some arbovirus.
However, the test is relatively cumbersome and time intensive (few days) relative to EIA kits that give quick results (usually several minutes to a few hours).
An issue with this assay that has recently been identified is that the neutralization ability of the antibodies is dependent on the virion maturation state and the cell-type used in the assay.[3] Therefore, if the wrong cell line is used for the assay it may seem that the antibodies have neutralization ability when they actually do not, or vice versa they may seem ineffective when they actually possess neutralization ability.[citation needed]
See also
References
- ^ Thomas, Stephen J.; Ananda Nisalak; Kathryn B. Anderson; Daniel H. Libraty; Siripen Kalayanarooj; David W. Vaughn; Robert Putnak; Robert V. Gibbons; Richard Jarman; Timothy P. Endy (November 2009). "Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance". The American Journal of Tropical Medicine and Hygiene. 81 (5): 825–833. doi:10.4269/ajtmh.2009.08-0625. ISSN 0002-9637. PMC 2835862. PMID 19861618.
- ^ Ratnam, S; V Gadag; R West; J Burris; E Oates; F Stead; N Bouilianne (1995-04-01). "Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody". J. Clin. Microbiol. 33 (4): 811–815. doi:10.1128/JCM.33.4.811-815.1995. PMC 228046. PMID 7790442.
- ^ Mukherjee, S.; Dowd, K. A.; Manhart, C. J.; Ledgerwood, J. E.; Durbin, A. P.; Whitehead, S. S.; Pierson, T. C. (2014). "Mechanism and Significance of Cell Type-Dependent Neutralization of Flaviviruses". Journal of Virology. 88 (13): 7210–7220. doi:10.1128/JVI.03690-13. ISSN 0022-538X. PMC 4054442. PMID 24741083.