Pulse-chase analysis
In microbiology, a pulse-chase analysis a method for examining a cellular process occurring over time by successively exposing the cells to a radioactive compound (pulse) and then to the same compound in nonradioactive form (chase).
Uses
This method is useful for determining the activity of certain cells over a prolonged period of time. The method has been used to study protein kinase C, ubiquitin, and many other proteins.