Trichoderma reesei
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Trichoderma reesei is a mesophilic and filamentous fungus. It is an anamorph of the fungus Hypocrea jecorina. T. reesei has the capacity to secrete large amounts of cellulolytic enzymes (cellulases and hemicellulases). Microbial cellulases have industrial application in the conversion of cellulose, a major component of plant biomass, into glucose.[1]
Recent advances in the biochemistry of cellulase enzymology, the mechanism of cellulose hydrolysis (cellulolysis), strain improvement, molecular cloning and process engineering are bringing T. reesei cellulases closer to being a commercially viable route to cellulose hydrolysis.[2] Several industrially useful strains have been developed and characterised, e.g. Rut-C30,[3] RL-P37 and MCG-80. The genome of this organism was released in 2008.[4] This organism also has a mating type dependent characterised sexual cycle.[5]
See also
- Cellobiohydrolase (CBH)
- Endoglucanase (EG)
- Cellulosic ethanol
References
External links
- Risk Assessment Summary, CEPA 1999. Trichoderma reesei 1391A
- Risk Assessment Summary, CEPA 1999. Trichoderma reesei P59G
- Risk Assessment Summary, CEPA 1999. Trichoderma reesei P210A
- Risk Assessment Summary, CEPA 1999. Trichoderma reesei P345A
- http://www.eere.energy.gov/cleancities/progs/afdc/vwbs2.cgi?200
- Trichoderma spp.
- from genencor DNA sequence and Trichoderma reesei EST Database and Mitochondrial Genome.
- Nidetzky B, Steiner W, Claeyssens M: Cellulose hydrolysis by the cellulases from Trichoderma reesei: adsorptions of two cellobiohydrolases, two endocellulases and their core proteins on filter paper and their relation to hydrolysis. In: Biochem. J. 303. Jahrgang, Pt 3, November 1994, S. 817–23, PMID 7980450, PMC 1137620 (freier Volltext).
- Miettinen-Oinonen A, Suominen P: Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric. In: Appl. Environ. Microbiol. 68. Jahrgang, Nr. 8, August 2002, S. 3956–64, doi:10.1128/AEM.68.8.3956-3964.2002, PMID 12147496, PMC 124001 (freier Volltext) – (asm.org).
- CBH I from Trichoderma sp..
- CBH I from Trichoderma sp..
- Medve J, Ståhlberg J, Tjerneld F: Adsorption and synergism of cellobiohydrolase I and II of Trichoderma reesei during hydrolysis of microcrystalline cellulose. In: Biotechnol. Bioeng. 44. Jahrgang, Nr. 9, November 1994, S. 1064–73, doi:10.1002/bit.260440907, PMID 18623023.
- ↑ Kumar R, Singh S, Singh OV: Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. In: J. Ind. Microbiol. Biotechnol. 35. Jahrgang, Nr. 5, Mai 2008, S. 377–91, doi:10.1007/s10295-008-0327-8, PMID 18338189.
- ↑ Viikari L, Alapuranen M, Puranen T, Vehmaanperä J, Siika-Aho M: Thermostable enzymes in lignocellulose hydrolysis. In: Adv. Biochem. Eng. Biotechnol. 108. Jahrgang, 2007, S. 121–45, doi:10.1007/10_2007_065, PMID 17589813.
- ↑ [Seidl V, Gamauf C, Druzhinina IS, Seiboth B, Hartl L, Kubicek CP: The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. In: BMC Genomics. 9. Jahrgang, 2008, S. 327, doi:10.1186/1471-2164-9-327, PMID 18620557, PMC 2483294 (freier Volltext) – (biomedcentral.com).
- ↑ Martinez D, Berka RM, Henrissat B, et al.: Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). In: Nat. Biotechnol. 26. Jahrgang, Nr. 5, Mai 2008, S. 553–60, doi:10.1038/nbt1403, PMID 18454138.
- ↑ [Seidl V, Seibel C, Kubicek CP, Schmoll M.: Sexual development in the industrial workhorse Trichoderma reesei. In: PNAS. 106. Jahrgang, Nr. 33, 2009, S. 13909–13914, doi:10.1073/pnas.0904936106, PMID 19667182, PMC 2728994 (freier Volltext).