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Automated Imaging Microscope System

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This is an old revision of this page, as edited by Sgaran (talk | contribs) at 18:28, 1 May 2010. The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.


Dear wikipedia colleagues, I would like to update you on a issue that has become disturbing to me. A few days ago Wim Crusio and I had a disagreement over the word "Phenomics" which has been used in many scientific publications and online dictionaries. Wim Crusio has been changing the wiki entry to re-direct it to the word "Phenotype" and I have tried to keep the word as a stand alone term. After his repeated failed attempts to redirect the word to "Phenotype" he has decided to carry out a reprehensible vendetta against myself, my work and my co-researcher who passed away in 2008. As you can see by Wim Crusio's edit history on the following items, he started to delete and alter the following items, on April 29, 2010, which was directly after our disagreement over the word "Phenomics" :

Steven A. Garan Aging Research Centre Automated Imaging Microscope System Paola S. Timiras

I hope the contributors to wikipedia do not encourage this kind of childish behavior. If Wim Crusio has a disagreement regarding an issue with any of my wikipedia colleagues, I would hope to stand by them should a person like Wim Crusio carry out a similar campaign. What I find utterly reprehensible is Wim Crusio's sudden interest in my co-researcher of ten years Paola S. Timiras. She passed away in September of 2008 and starting on April 29, 2010, his actions in altering her page are clearly an act driven not my any scientific motivation, but instead by a malicious desire for revenge.

Steven A. Garan



The Automated Imaging Microscope System (AIMS) was developed by scientists at the University of California, Berkeley and the Aging Research Centre (ARC). Steven A. Garan, was the lead scientists that developed the AIMS system along with Warren Freitag, Jason Neudorf and members of the UC Berkeley lab where AIMS was developed and utilized. Many journals articles have been published about the system and the results that it produced. Since the completion of the first version in 1998, newer versions were developed, with the final version being completed in 2007. Empowering investigators to accurately count specific cell populations is essential to all fields of neurobiology. While computer assisted counting technology has been in use for over a decade, advances in an Automated Imaging Microscope System (AIMS), now insure 97% accuracy when comparing computer counts to human counts for both nuclear and cytoplasmic stained tissue. More importantly, regional analysis can now be customized so that only cell populations within specified anatomic regions will be targeted for counting, thus reducing the background noise of non-immunoreactive cells when characterizing specific cell populations. This application was recently used to successfully map the density and distribution of both nuclear expressed estrogen receptor-alpha and cytoplasmicly expressed IGF-1 receptor in specific hypothalamic nuclei. Furthermore, AIMS can now detect intra-hypothalamic differences in receptor expression and measure phenomenon such as lateralization. By using this technology, the evaluation of tissue-level biology can be used to establish neuroendocrine biomarkers of aging, and analyze the neuroendocrine effects of caloric restriction and gene knockout models that extend the lifespan.

Publications

  • Yaghmaie F, Saeed O, Garan SA, Voelker MA, Sternberg H, Timiras PS (February 2010). "Estrogen receptor-alpha immunoreactivity in the arcuate hypothalamus of young and middle-aged female mice". Neuro Endocrinology Letters 31 (1): 15. PMID 20150877.
  • Gouw, A; Mahuron, K; Manandhar, S; Tin, A; Garan, S; Hakimi, P; Timiras, P (2009). "The plasticity of neural cell reprogramming: Role of growth factors in inducing neuroglia to neuron and to neural precursor cell". Experimental Gerontology 44: 129. doi:10.1016/j.exger.2008.08.025.
  • Gouw AM, Panchal H, Mahuron K, Wadhwani H, Manandhar S, Garan SA, Tin A, Timiras PS (June 15, 2008). "The Potential Role of Epidermal Growth Factor (EGF) and Curcumin in Transforming Neuroglia into Neurons". The Endocrine Society 90th Annual Meeting. San Francisco, CA. Retrieved February 27, 2010.
  • Garan, S; Yaghmaie, F; Saeed, O; Gouw, A; Freitag, W; Voelker, M; Jafar, P; Kaur, J et al. (2007). "Novel methods in computer-assisted tissue analysis: Customized regional targeting of both cytoplasmic and nuclear-stained tissue". Experimental Gerontology 42: 141–2. doi:10.1016/j.exger.2006.06.015.
  • Yaghmaie F, Saeed O, Garan SA, et al. (November 2006). "Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice". International Journal of Developmental Neuroscience 24 (7): 431–6. doi:10.1016/j.ijdevneu.2006.08.008. PMID 17034982.
  • Yaghmaie, F; Saeed, O; Garan, S; Gouw, A; Jafar, P; Kaur, J; Nijjar, S; Timiras, P et al. (2007). "Tracking changes in hypothalamic IGF-1 sensitivity with aging and caloric restriction". Experimental Gerontology 42: 148–9. doi:10.1016/j.exger.2006.06.043.
  • Saeed O, Yaghmaie F, Garan SA, et al. (February 2007). "Insulin-like growth factor-1 receptor immunoreactive cells are selectively maintained in the paraventricular hypothalamus of calorically restricted mice". International Journal of Developmental Neuroscience 25 (1): 23–8. doi:10.1016/j.ijdevneu.2006.11.004. PMID 17194562.
  • Yaghmaie F, Saeed O, Garan SA, et al. (November 2006). "Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice". International Journal of Developmental Neuroscience 24 (7): 431–6. doi:10.1016/j.ijdevneu.2006.08.008. PMID 17034982.
  • Yaghmaie, Farzin; Saeed, Omar; Garan, Steven A.; Gouw, Arvin M.; Tran, Tien; Ho, Jacqueline; Zhao, Liu Y.; Voelker, Mark A. et al. (March 2006). "A Study of Insulin-like Growth Factor-I Receptor Immunoreactivity in the Supraoptic Nucleus of Young and Old Female B6D2F1 Mice". The FASEB Journal 20 (4): A536.
  • Yaghmaie F, Saeed O, Garan SA, Freitag W, Timiras PS, Sternberg H (June 2005). "Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice". Neuro Endocrinology Letters 26 (3): 197–203. PMID 15990721.
  • Yaghmaie F, Garan SA, Saeed O, Freitag W, Timiras PS, Sternberg H, Voelker M (November–December 2004). "A survey of estrogen receptor-alpha immunoreactivity in the hypothalamus of young, old, and old-calorie restricted female B6D2F1 mice". Experimental Gerontology 39 (11–12): 1771.
  • Voelker M, Yaghmaie F, Garan SA, Sternberg H (November–December 2004). "Protocol for higher resolution histological images of the mammalian brain". Experimental Gerontology 39 (11–12): 1770.
  • Yaghmaie F, Garan SA, Massaro M, Timiras PS (January 2003). "A comparison of estrogen receptor-alpha immunoreactivity in the arcuate hypothalamus of young and middle-aged C57BL6 female mice". Experimental Gerontology 38 (1–2): 220.
  • Garan SA, Neudorf J, Tonkin J, McCook LR, Timiras PS (December 2000). "Creating Three-Dimensional Neuronal Maps of the Mouse Hypothalamus Using an Automated Imaging Microscope System". Experimental Gerontology 35 (9–10): 1421.
  • Neudorf, Jason; Garan, Steven A. (February 2000). "Automated Imaging Microscope System". Linux Journal (70): 32–5.


See also


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